Open AccessDevelopment of microsatellite markers for two Australian Persoonia (Proteaceae) species using two different techniques
Author(s) -
Stingemore Jessica A.,
Nevill Paul G.,
Gardner Michael G.,
Krauss Siegfried L.
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1300023
Subject(s) - biology , microsatellite , genetic diversity , population , evolutionary biology , transferability , genetic variation , zoology , genetics , allele , gene , statistics , demography , mathematics , logit , sociology
• Premise of the study: Microsatellite markers were developed and cross‐species transferability assessed for two Persoonia species to evaluate genetic diversity and population genetic structure of these broadly distributed southwest Australian tree species. • Methods and Results: Microsatellite‐enriched libraries and 454 GS‐FLX shotgun sequencing were used to identity nine microsatellite loci for P. elliptica (one 454; eight cloning) and six for P. longifolia (three 454; three cloning). These loci were screened for variation in individuals from populations in southwestern Australia. In P. elliptica , observed and expected heterozygosities ranged from 0.46 to 0.93 and 0.42 to 0.88, respectively. For P. longifolia , observed and expected heterozygosities ranged from 0.04 to 0.88 and 0.04 to 0.84, respectively. • Conclusions: The microsatellites identified in this study will enable the examination of population and spatial structuring of genetic diversity in P. elliptica and P. longifolia , two priority species for mine site restoration in southwestern Australia.