Open AccessDevelopment of microsatellite markers in the oil‐producing species Vernicia fordii (Euphorbiaceae), a potential biodiesel feedstock
Author(s) -
Pan Yue,
Pan Lei,
Chen Liang,
Zhang Lingling,
Nevo Eviatar,
Peng Junhua
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1200004
Subject(s) - biology , microsatellite , germplasm , genetic marker , molecular breeding , molecular marker , genetic diversity , euphorbiaceae , botany , amplified fragment length polymorphism , genetics , gene , allele , demography , sociology , population
• Premise of the study: Tung tree, Vernicia fordii , is native to China. Little has been done on genetics and breeding at the molecular level in this species, let alone utilizing microsatellite (simple sequence repeat [SSR]) markers. Therefore, a set of SSR molecular markers was developed for studies on molecular genetics and breeding in tung tree. • Methods and Results: We designed 78 SSR markers using a protocol based on the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Assessed in 81 V. fordii accessions, 40 of these markers were polymorphic and 12 of them showed monomorphism. When tested using six V. montana accessions, 52 of the markers were capable of PCR amplification and 25 were polymorphic. • Conclusions: The newly developed SSR markers are effective and helpful in the evaluation of genetic germplasm and molecular breeding in tung tree.