Open AccessSingle Nucleotide Polymorphisms in the Promoter of CYP19 Gene in Cattle Bred in Iraq
Author(s) -
Salah H. Faraj,
Asaad Y. Ayied,
Khalaf A. H. Al-Rishdy
Publication year - 2020
Publication title -
maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2520-0860
pISSN - 1814-5868
DOI - 10.37077/25200860.2020.33.1.07
Subject(s) - haplotype , single nucleotide polymorphism , genetics , genbank , biology , nucleotide diversity , gene , genetic diversity , phylogenetic tree , snp , promoter , genotype , population , gene expression , medicine , environmental health
The present study was undertaken to characterize the genetic diversity of the aromatase cytochrome P450 (CYP19) gene in 34 cows (15 local, 14 Holstein, and 5 Crosses) in Iraq. The objectives of the present study are to detect SNPs (mutations) in promoter p1.1 of the CYP19 gene in cattle bred in Iraq using sequencing techniques. We identified five single-nucleotide polymorphisms (SNP) loci of the CYP19 gene that were detected, namely G933T, G994C, A1044G, A1062T, and C1468A. The results showed the presence of 3, 4, and 2 polymorphic sites leading to the construction of 4, 5, and 3 different haplotypes for Holstein, local, and crosses respectively. Haplotype diversity were 0.791, 0.752, and 0.700 respectively. While nucleotide diversity was 0.0017, 0.0022, and 0.0013 respectively. Besides, we carried out a phylogenetic analysis of these sequences to address the evolutionary relationship between the animal species. These fragments were assigned in the GenBank database under the accession numbers: LC490756, LC490757, LC491437, LC491438, LC491439, LC491588, and LC491589.