Open AccessThe interacting effects of genetic variation in Geranium subg. Geranium (Geraniaceae) using scot molecular markers
Author(s) -
Bo Shi,
Majid Khayatnezhad,
Abdul Shakoor
Publication year - 2021
Publication title -
caryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.319
H-Index - 29
eISSN - 2165-5391
pISSN - 0008-7114
DOI - 10.36253/caryologia-1079
Subject(s) - geranium , biology , genetic diversity , geraniaceae , genetic variation , botany , genetic distance , mantel test , genetics , population , gene , cultivar , demography , sociology
One of the most crucial aspects of biological diversity for conservation strategies is genetic diversity, particularly in rare and narrow endemic species. Our study is the first attempt to utilize SCoT markers to check the genetic diversity in Iran. We used 115 plant samples. Our objectives were 1) to check genetic diversity among Geranium species 2) Genetic structure of the Geranium 3) Do the Geranium species exchange genes? 4) To detect isolation by distance among the Geranium species. We used traditional morphological and molecular methods to assess genetic diversity and genetic structure in the Geranium species. A total of 129 amplified polymorphic bands were generated across 13 Geranium species. The size of the amplified fragments ranged from 150 to 3000 bp. G. stepporum showed the highest values for the effective number of alleles (Ne = 1.30) and Shannon information index (I =0.35). Significant ANOVA results (P <0.01) showed differences in quantitative morphological characters in plant species. G. sylvaticum showed high genetic diversity. Mantel test showed a significant correlation (r = 0.17, p=0.0002) between genetic distance and geographical distance, so isolation by distance (IBD) occurred among the Geranium species. According to the SCoT markers analysis, G. kotschyi and G. dissectum had the lowest similarity, and the species of G. sylvaticum and G. pratense had the highest similarity. The present study revealed that a combination of morphological and SCoT methods could distinguish the species of Geranium.