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Studies of the epidemiology of arthropod-borne viruses are based on understanding whether a given virus is found in a particular location or locations, how prevalent the virus is in that area, which vertebrate hosts serve as reservoirs of the virus, the relationship between the size of the populations of these reservoirs and the prevalence of the virus and, of course, whether the virus causes human, livestock, or wildlife diseases, as well as other characteristics. However, seasonal conditions fluctuate, annual conditions fluctuate, human impacts alter the environment, and the habitat and ecosystems naturally change. Because these parameters provide a more encompassing view of the natural history of a virus, it is important to recognize that point prevalence studies comprise only a single view of the situation and that longitudinal studies are required to obtain a more complete and useful understanding of the natural history of the virus. This paper provides details as to whether and how to conduct such studies.

Point Prevalence Studies Are Necessary as First Steps in Studying the Epidemiology of Viruses and Other Pathogens Transmitted by Arthropods