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Background: The use of knock-out mouse models is crucial to understand platelet activation and aggregation. Methods: Analysis of the global double fluorescent Cre reporter mouse  mT/mG  that has been crossbred with the megakaryocyte/platelet specific  PF4-Cre  mouse. Results: Platelets show bright  mT  ( PF4-Cre  negative) and  mG  ( PF4-Cre  positive) fluorescence. However, a small proportion of leukocytes was positive for  mG  fluorescence in  PF4-Cre  positive mice. In  mT/mG;PF4-Cre  mice, platelets, and megakaryocytes can be tracked by their specific fluorescence in blood smear, hematopoietic organs and upon thrombus formation. No differences in platelet activation and thrombus formation was observed between  mT/mG;PF4-Cre  positive and negative mice. Furthermore, hemostasis and in vivo thrombus formation was comparable between genotypes as analyzed by intravital microscopy. Transplantation studies revealed that bone marrow of  mT/mG;PF4-Cre  mice can be transferred to  C57BL/6  mice. Conclusions: The  mT/mG Cre  reporter mouse is an appropriate model for real-time visualization of platelets, the analysis of cell morphology and the identification of non-recombined platelets. Thus,  mT/mG;PF4-Cre  mice are important for the analysis of platelet-specific knockout mice. However, a small proportion of leukocytes exhibit  mG  fluorescence. Therefore, the analysis of platelets beyond hemostasis and thrombosis should be critically evaluated when recombination of immune cells is increased.

Genetic Labeling of Cells Allows Identification and Tracking of Transgenic Platelets in Mice