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microRNAs are a kind of endogenous, non-coding, single-strand small RNA. They have been reported as an important regulatory factor in skeletal myogenesis. In this study, miR-452 was selected from RNA high-throughput sequencing data to explore its regulatory role in myogenesis. Functionally, miR-452 overexpression could promote C2C12 myoblast proliferation while inhibiting myogenic differentiation. On the contrary, inhibition of miR-452 could suppress C2C12 myoblast proliferation but accelerate myogenic differentiation. Bioinformatics analysis and dual luciferase report assays showed that  Angiopoietin 1  ( ANGPT1 ),  RB1 , and  CACNB4  were the potential target genes of miR-452. To further confirm the target relationship between  ANGPT1 ,  RB1 , and  CACNB4  with miR-452, the mRNA level and protein level of these genes were detected by using RT-qPCR and Western blot, respectively. Result analysis indicated that  ANGPT1  was a target gene of miR-452. In addition, knockdown of  ANGPT1  could obviously promote C2C12 myoblast proliferation but block their differentiation. In summary, these results demonstrated that miR-452 promoted C2C12 myoblast proliferation and inhibited their differentiation  via  targeting  ANGPT1 .

MiR-452 Regulates C2C12 Myoblast Proliferation and Differentiation via Targeting ANGPT1