Open AccessHigh Performance Liquid Chromatographic Method for Determination of Ascorbic Acid in Pure Form, Pharmaceutical Formulations, Biological Samples and in Environmental Water Samples
Author(s) -
Nief Rahman Ahmed,
karam Basil
Publication year - 2014
Publication title -
almustansiriya journal of pharmaceutical sciences/al-mustansiriyah journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2959-183X
pISSN - 1815-0993
DOI - 10.32947/ajps.v14i1.132
Subject(s) - ascorbic acid , chromatography , detection limit , calibration curve , chemistry , high performance liquid chromatography , dosage form , methanol , organic chemistry , food science
A simple, precise, rapid, and accurate reversed–phase high performance liquidchromatographic method has been developed for the determination of ascorbic acid in purefrom, pharmaceutical formulations, biological samples and in environmental water samples.Chromatography was carried out on supercar L1 (C18 ) reversed- phase column (25cm×4.6mm), 5 microns, using a mixture of methanol : water (42: 58v/v) as a mobile phase at aflow rate of 1.0 ml.min-1.Detection was performed at 254nm at ambient temperature.The retention time for ascorbic acid was found 2.3 minutes. The calibration curve waslinear (r= 0.9998) over a concentration range from 0.5 to 12.5μg/ml. Limit of detection(LOD) and limit of quantitation (LOQ) were found 3ng/ml and 9 ng/ml respectively. Themethod was validated for its linearity, precision and accuracy.The proposed method was successfully applied for the determination of ascorbic acidin pure form, pharmaceutical formulations, and biological samples and in environmentalwater samples.