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Seven  in absentia  (SINA) family proteins play a central role in plant growth, development and resistance to abiotic stress. However, their biological function in plant response to cold stress is still largely unknown. In this work, a  seven in absentia  gene  IbSINA5  was isolated from sweet potato. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses demonstrated that  IbSINA5  was ubiquitously expressed in various tissues and organs of sweet potato, with a predominant expression in fibrous roots, and was remarkably induced by cold, drought and salt stresses. Subcellular localization assays revealed that IbSINA5-GFP fusion protein was mainly localized in cytoplasm and nucleus. Overexpression of  IbSINA5  in sweet potato led to dramatically improved resistance to cold stress in transgenic plants, which was associated with the up-regulated expression of  IbCOR  (cold-regulated) genes, increased proline production, and decreased malondialdehyde (MDA) and H 2 O 2  accumulation in the leaves of transgenic plants. Furthermore, transient expression of  IbCBF3 , a C-repeat binding factor (CBF) gene, in the leaf protoplasts of wild type sweet potato plants up-regulated the expression of both  IbSINA5  and  IbCOR  genes. Our results suggest that  IbSINA5  could function as a positive regulator in the cold signaling pathway through a CBF-SINA-COR mediated module in sweet potato, and have a great potential to be used as a candidate gene for the future breeding of new plant species with improved cold resistance.

Overexpression of IbSINA5 Increases Cold Tolerance through a CBF SINA-COR Mediated Module in Sweet Potato