English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Rho-related GTPase from plants (ROP) proteins play an essential role in plant stress resistance. In this study, the full-length  GlROP6  gene was cloned based on  G. littoralis  transcriptome sequencing data acquired in response to salt stress. The protein sequence, conserved domains, secondary structure, three-dimensional structure, phylogenetic relationships, and expression pattern of the  GlROP6  gene were systematically analysed. Our results showed that the full-length  GlROP6  gene had an open reading frame of 606 bp, which encoded 201 amino acid residues with a relative molecular weight of 22.23463 kDa and a theoretical isoelectric point of 9.06. Amino acid sequence analyses indicated that the structure of the GlROP6 protein was conserved, and included five G-box motifs (G1–G5), an effector binding region, a Rho insert region and a C-terminal hypervariable region. According to our phylogenetic analysis, the GlROP6 protein was closely related to the ROP protein of  Daucus carota  subsp.  Sativus . Our quantitative real-time PCR results revealed that  GlROP6  was highly expressed in flower, and  GlROP6  expression was significantly upregulated in  G. littoralis  roots treated with NaCl. This study will facilitate investigations into the function of  GlROP  genes in response to salt stress in  G. littoralis .

Cloning and Bioinformatics Analysis of the GlROP6 gene in Glehnia littoralis