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Cinnamomum japonicum  Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study, we successfully developed a large-scale micropropagation protocol for  C. japonicum  for the first time. Sterilized shoots were excised and used as explants for shoot induction on several basal media, supplemented with different concentrations of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N 6 -Benzyladenine (6-benzylaminopurine) (BA),  α-naphthaleneacetic  acid (NAA) and Gibberellic acid (GA 3 ). After comparison, the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L –1  BA, 0.05 mg L –1  NAA and 0.2 mg L –1  GA 3 , which resulted in an average number of induced shoots per explant and shoot length of 5.2 and 1.62 cm at 28 d, respectively. Then, elongated adventitious shoots were transferred to induce roots. 86.7% of shoots was able to root on 1/2 MS medium supplemented with 0.5 mg L –1  NAA and 0.1 mg L –1  BA. The earliest rooting time observed was after 21 d and the average root length was up to 3.3 cm after 28 d. Our study shows that  C. japonicum  can be successfully regenerated through  de novo  organogenesis, which lays a foundation for future transformation research on this tree.

Efficient Evergreen Plant Regeneration of Cinnamomum japonicum Sieb. through in vitro Organogenesis