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The rapid spread of the SARS-CoV-2 virus and increased number of deaths urged the world to provide accurate diagnostic procedures and apply the correct prevention measures to eradicate the disease. Molecular assays are designed to test the presence of virus RNA. The assays have wanting accuracy represented by false positive and false negative results, in particularly with continuous emerging of SARS-CoV-2 genetic variability. Miss interpretation of the test results can lead to bad outcomes for both individuals and the surrounding community when assuming the test is perfect. This review provides an update of the currently available molecular laboratory methods and address the effect of ongoing SARS-CoV-2 genetic variability on the performance of RT-PCR, the problem of re-positive test result in recovered patients and provides elements to be considered for performing SARS-CoV-2 test and interpreting the test results.

Accuracy of SARS-CoV-2 molecular laboratory testing: insight on recurrence infection and the new emergent strains