Open AccessIsolasi Dan Karakterisasi Lipase Dari Rebung Bambu Betung Dendrocalamusasper
Author(s) -
Agung Prasetyo,
Winni Astuti,
Chairul Saleh
Publication year - 2017
Publication title -
jurnal kimia mulawarman
Language(s) - English
Resource type - Journals
eISSN - 2476-9258
pISSN - 1693-5616
DOI - 10.30872/jkm.v15i1.585
Subject(s) - lipase , bamboo , bamboo shoot , chromatography , chemistry , centrifugation , enzyme , biology , botany , biochemistry , organic chemistry , raw material
This research was conducted to get the extract of rough lipase produced by bamboo shoots (Dendrocalamus asper). Lipase isolation from bamboo shoots was done by blending bamboo shoots to produce the crude extract of the enzyme. Lipase crude extracts that occurred at centrifugation at 12000 rpm for 30 minutes at 4°C. The crude extracts obtained were then tested using quantitative pH 7, temperature 30ºC and substrate 1% (v/v). Based on research there is activity of crude extract of lipase equal to 2,24 U/ml.