Open AccessOPTIMIZATION OF NEUROTRANSMITTERS SEPARATION UNDER HILIC CONDITIONS
Author(s) -
Raluca Tampu,
Cătălin Tâmpu
Publication year - 2017
Publication title -
journal of engineering studies and research
Language(s) - English
Resource type - Journals
eISSN - 2344-4932
pISSN - 2068-7559
DOI - 10.29081/jesr.v23i1.90
Subject(s) - ammonium formate , acetonitrile , hydrophilic interaction chromatography , chromatography , chemistry , ammonium , salt (chemistry) , chromatographic separation , phase (matter) , formate , high performance liquid chromatography , organic chemistry , catalysis
This paper presents the optimization of a hydrophilic interaction chromatographic method for the separation of eleven catecholamines, indolamines and their precursors and metabolites. The studied parameters are: the organic modifier nature and percentage, the salt nature and concentration, the mobile phase pH and the column temperature. The best results in terms of separation were obtained using a mobile phase composed of acetonitrile and ammonium formate (150 mM pH 3) 85:15 v:v.