Open AccessQuantification of bioactive compounds in guava at different ripening stages
Author(s) -
Shrirama Shukla,
R. K. Kushwaha,
Mohini Singh,
Ravi Saroj,
Vinita Puranik,
R. Agarwal,
Dalbir Kaur
Publication year - 2021
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.5(3).554
Subject(s) - psidium , gallic acid , ascorbic acid , ellagic acid , ripening , myricetin , myrtaceae , chemistry , quercetin , cultivar , food science , lycopene , bioactive compound , polyphenol , antioxidant , horticulture , botany , biology , carotenoid , kaempferol , biochemistry
Guava (Psidium guajava L.) is one of the most important tropical fruits belonging tothe genus Psidium and the Myrtaceae family and claim to have phenolic compoundsthat have been reported to possess strong antioxidant activity. This study was aimed toevaluate the bioactive constituents in guava cultivars at different ripening stages byHPLC. The five guava cultivars were selected at different ripening stages and thebioactive components were analysed by high-pressure liquid chromatography. Thequantification of bioactive compounds revealed that the highest amount of bioactivecompounds was found in cultivar Safeda at the unripe stage, while a minimum amountwas found in ripe Apple Colour guava cultivar. The six bioactive compounds werequantified in the range of gallic acid (9.46-63.08 mg/100 g), quercetin (0.11-2.51mg/100 g), myrcetin (0.09-0.034 mg/100 g), ascorbic acid (7.45-75.07 mg/100 g),apegenin (0.01-0.032 mg/100 g) and lycopene (0.34-0.92 mg/100 g). The exploratoryevaluation of guava samples was performed through Principal Component Analysis(PCA), the bioactive compounds, lycopene, myricetin, and quercetin are dominantvariables on this PC1 (61.52%) (Scores better than 0.7), thereby causing greatervariability among these samples. The second principal component (PC2) represents16.54% of the total variance and the ascorbic acid, gallic acid and apeginin (scorebetter than 0.7), are the dominant variables on this PC.