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The response of low-density graphite electrodes hosting Trametes hirsuta laccase in a direct electron transfer regime is presented for real-time analysis of O 2  concentrations. The use of contrasting immobilisation methods developed for biocathodes affords good reproducibility and reliability of the amperometric biosensor, which shows a limit of detection below 1 μM and a sensitivity slightly higher than 60 nA cm −2  M −1 .

Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode