Open AccessOptimization, Production and Antifungal Activity of Chitinase Produced by Trichoderma harezianum
Author(s) -
Qays M. Issa
Publication year - 2016
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2016.10.1.451
Subject(s) - chitinase , incubation , trichoderma , chitin , incubation period , microbiology and biotechnology , antifungal , biology , chemistry , food science , botany , enzyme , biochemistry , chitosan
Twelve of Trichoderma spp. Isolates were obtained from 30 soils samples collected from differentcrops fields in Baghdad city. Among them, isolate designated as TLT4 which produced highestchitinase activity in primary and secondary screenings was identified as Trichoderma harezianum.The high level of chitinase production was observed in chitinase production media (CPM)amended with 0.4% colloidal chitin with pH6, incubation temperature 30°C, incubation period 6days, peptone as nitrogen source and inoculum size 1× 105spore/ml using submerged cultures.The antifungal activity of crude T. harezianumchitinase was investigated against fourdermatophytes ncluding: Trichophytonment agrophytes, T. rubrum, Microsporumcanis, M.gypsum. The crude chitinase was found to inhibit the growth of all dermatophytes tested withvarying degrees. Clotrimazole drug was used to compare.