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Leaf spot disease caused by  Alternaria helianthi  (Hansf.) is an important fungal disease of sunflower in India and elsewhere. Non-availability of known sources for resistance to Alternaria is a major constraint in sunflower breeding. Hence, seeds of sunflower genotypes were subjected to gamma-irradiation to develop mutant populations. Mutants were selected from the M2 generation based on percent disease incidence and were passed on to the M3 generation. Genomic DNA from leaf samples was evaluated by PCR amplification using random decamer primers and the amplified DNA fragments from mutants were compared with their respective controls. Single marker and stepwise regression analysis carried out in relation to percent disease index indicate that the alleles OPC5-B, K, J, OPA12-D and OPA15-A are strongly associated with  Alternaria helianthi  resistance.

helia

ASSESSMENT OF HERITABILITY OF Alternaria helianthi RESISTANCE TRAIT IN SUNFLOWER USING MOLECULAR MARKERS / EVALUACIÓN DE LA HERENCIA DE RESISTENCIA A Alternaria helianthi EN GIRASOL UTILIZANDO LOS MARCADORES MOLECULARES / ESTIMATION DE L’APTITUDE DU TOURNESOL À HÉRITER DE LA RÉSISTANCE À l’Alternaria helianthi AU MOYEN DES MARQUEURS MOLÉCULAIRES