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By using the direct organogenesis method, applied for the first time at the Biotechnology Laboratory of DAI, General Toshevo, new sunflower forms were obtained through distant hybridization from the cross  H .  annuus  L. (cv. Albena) ×  Helianthus tuberosus  (Encheva  et al ., 1992). Three to eight hybrid plants were produced from a single embryo through the direct organogenesis method. This is a valuable method because it allows to obtain more than one plant from a hybrid embryo, something which is impossible with the commonly used “ embryo rescue ” technique. A considerable number of new sunflower lines were produced after self-pollination and individual selection. After characterization of the hybrid progenies according to seventeen morphological and biochemical indices, the conclusion can be drawn that lines R101 and R104 show 58.8% and 70.6%, respectively, intermediate phenotype in comparison with the two parental forms. The positive transgressive forms were 29.4% and 23.5%, respectively, and the negative transgressive forms were 5.9% for both lines. Some of the R lines were directly included in heterosis breeding of sunflower.

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CHARACTERIZATION OF INTERSPECIFIC HYBRIDS BETWEEN CULTIVATED SUNFLOWER H. annuus L. (cv. ALBENA) AND WILD SPECIES Helianthus tuberosus / CARACTERIZACIÓN DE HÍBRIDOS INTERESPECIES ENTRE EL GIRASOL H. annuus L. (cv. ALBENA) CULTIVADO Y LA ESPECIE Helianthus tuberosus / CARACTÉRISATION DES HYBRIDES INTERSPECIES ENTRE LE TOURNESOL CULTIVÉ H. annuus L. (cv. ALBENA) ET L’ESPÈCE Helianthus tuberosus