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This work aims to clone the genomic region of the sunflower genome that may carry the Pervenets mutation causing high oleic content of seed oil. We previously reported that the oleHOS allele at the oleate-desaturase locus (oleHL) carries or is genetically closely linked to Pervenets mutation. We pointed out that the fragments that border the 5.8 kb  Eco RI fragments carry the Pervenets mutation. The oleHOS reference physical map was constructed. It displays a common part made of the 5.8 kb  Eco RI fragment, also present in oleLOR allele, and a specific region with the 7.9 kb  Eco RI fragment, also carrying oleate-desaturase sequences.

helia

REPEATS OF AN OLEATE DESATURASE REGION CAUSE SILENCING OF THE NORMAL GENE EXPLAINING THE HIGH OLEIC PERVENETS SUNFLOWER MUTANT / EXPLICACION DE LA MUTACION CON ALTA OLEINA EN PERVENEC A BASE DEL EFECTO DE AREAS REPETIDAS DE OLEATO-DESATURASE SOBRE LA INHIBICION DEL GEN NORMAL / EXPLICATION DE LA FORTE MUTATION OLÉIQUE DANS LE PERVENETS PAR L’EFFET D’INHIBITION DES RÉPÉTITIONS DE LA RÉGION OLÉATE SATURASE SUR LE GÈNE NORMAL