Open AccessCan C-phycocyanin be used as a sperm protector?
Author(s) -
Emma Gabriela Antonio Marcos,
O. Monroy,
E. Cortés Barberena,
Ernesto Rodríguez Tobón,
José Gregório Martínez,
Edith Arenas Ríos
Publication year - 2019
Publication title -
veterinaria méxico oa
Language(s) - English
Resource type - Journals
ISSN - 2448-6760
DOI - 10.22201/fmvz.24486760e.2018.4.642
Subject(s) - sperm , extender , oxidative stress , antioxidant , phycocyanin , andrology , motility , sperm motility , dna damage , reactive oxygen species , chemistry , biology , biochemistry , food science , microbiology and biotechnology , dna , genetics , medicine , cyanobacteria , bacteria , polyurethane , organic chemistry
C-phycocyanin (C-PC) is a biocompound extracted from Arthrospira maxima. It is a chromophore-protein complex, with antioxidant properties. Its ability to prevent oxidative stress allows for diverse medical applications. This study evaluates the use of C-PC as a protein supplement and an antioxidant for in-vitro sperm preservation in a short-term extender. Viability, progressive motility, DNA damage and percentage of reactive oxygen species where assessed in Swine (Sus scrofa domesticus) sperm stored for up to 72 hours at 4 °C. Treatments with C-PC had the following concentrations: 0, 34.5, 69, 138 and 207 μg mL−1. Progressive motility and percentage of sperm with undamaged DNA were unchanged (20%) after storage for 48 hours using the 138 μg mL C-PC concentration−1.