Open AccessParvovirus B19 affects thrombopoietin and IL-11 gene expression in human bone marrow mesenchymal stem cells
Author(s) -
Mahin Behzadi Fard,
Amir Atashi,
Sara Amiri,
Saeid Kaviani,
Mohammad Ali Gholampour,
Mansoureh Ajami
Publication year - 2021
Publication title -
future virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.462
H-Index - 34
eISSN - 1746-0808
pISSN - 1746-0794
DOI - 10.2217/fvl-2021-0020
Subject(s) - mesenchymal stem cell , bone marrow , haematopoiesis , stromal cell , thrombopoietin , transfection , parvovirus , biology , immunology , stem cell , cancer research , gene , virus , microbiology and biotechnology , genetics
Aim: Human bone marrow mesenchymal stem cells (hBMSCs) may be infected by parvovirus B19 (B19V). hBMSCs support bone marrow hematopoiesis by producing stromal cells, secretion of cytokines and growth factors, etc. Because of the lifetime persistent infection of the virus in healthy individual’s bone marrow, this study aims to evaluate B19V effects on hBMSCs gene expression of some crucial hematopoietic cytokines. Materials & methods: hBMSCs were transfected with pHI0 plasmid containing the B19V genome. The quantitative mRNA expression of target genes was evaluated 24 h after transfection. Results: Our findings demonstrated a significant increase in expression levels of IL-11 and TPO (p < 0.05). Conclusion: We concluded that alteration in the gene expressions in B19V-infected hBMSCs might have significant effects on the bone marrow microenvironment as well as hematopoiesis.