Open AccessGenetic Polymorphism of Growth Hormone Gene Exon-4 in Surti and Mehsani Goats by PCR- RFLP
Author(s) -
Jyotishree Bayan,
V. B. Kharadi,
Umed Ramani,
Mamta Janmeda,
K. K. Tyagi,
Nikhil Dangar,
Gaurav Pandya
Publication year - 2018
Publication title -
the indian journal of veterinary sciences and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2395-1176
pISSN - 2394-0247
DOI - 10.21887/ijvsbt.v14i1.12993
Subject(s) - haeiii , restriction fragment length polymorphism , genotype , biology , exon , polymerase chain reaction , polymorphism (computer science) , gene , allele , genetics , restriction enzyme , microbiology and biotechnology
The present investigation was planned to study growth hormone (GH) gene exon-4 polymorphism using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Surti and Mehsani goats. GH gene exon-4 region was found to be monomorphic on restriction digestion with HaeIII, which revealed only one genotype CC in both Surti and Mehsani goat breeds. The allelic frequency of C was 1.00 in both the breeds of goats with absence of D allele.