Open Access<p>Cas9 Mediated Correction of β-<em>catenin</em> Mutation and Restoring the Expression of Protein Phosphorylation in Colon Cancer HCT-116 Cells Decrease Cell Proliferation in vitro and Hamper Tumor Growth in Mice in vivo</p>
Author(s) -
Yanlan Li,
Xiangning Li,
Jiayao Qu,
Dixian Luo,
Zheng Hu
Publication year - 2020
Publication title -
oncotargets and therapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.054
H-Index - 60
ISSN - 1178-6930
DOI - 10.2147/ott.s225556
Subject(s) - crispr , cas9 , survivin , cancer research , mutation , microbiology and biotechnology , cell growth , transfection , biology , colorectal cancer , catenin , cancer , cell culture , gene , wnt signaling pathway , genetics
Colorectal cancer (CRC) is one of the major contributors to cancer mortality and morbidity. Finding strategies to fight against CRC is urgently required. Mutations in driver genes of APC or β-catenin play an important role in the occurrence and progression of CRC. In the present study, we jointly apply CRISPR/Cas9-sgRNA system and Single-stranded oligodeoxynucleotide (ssODN) as templates to correct a heterozygous ΔTCT deletion mutation of β-catenin present in a colon cancer cell line HCT-116. This method provides a potential strategy in gene therapy for cancer.