Open Access<p>Expression and significance of LncRNA MNX1-AS1 in non-small cell lung cancer</p>
Author(s) -
Gaofeng Liu,
Xiaofeng Guo,
Yong Zhang,
Yan Liu,
Dandan Li,
Guangyao Tang,
Sujuan Cui
Publication year - 2019
Publication title -
oncotargets and therapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.054
H-Index - 60
ISSN - 1178-6930
DOI - 10.2147/ott.s198014
Subject(s) - gene knockdown , cancer research , lung cancer , cell growth , apoptosis , a549 cell , oncology , biology , medicine , cell culture , biochemistry , genetics
Objective: To investigate the expression of LncRNA MNX1-AS1 in NSCLC and its effect on NSCLC cell lines. Methods: In this experiment, the expression of LncRNA MNX1-AS1 was detected by qRT-PCR in 116 NSCLC samples, and the correlation between MNX1-AS1 and NSCLC patients was further analyzed by chi-square test. The prognostic value of MNX1-AS1 was assessed by Kaplan-Meier survival curve. The expression of MNX1-AS1 in NSCLC cell line A549 was knocked down, and the effects of MNX1-AS1 on proliferation, apoptosis, migration and invasion of NSCLC cells were evaluated. Results: Compared with normal lung tissue, the expression of MNX1-AS1 was significantly increased in lung cancer tissues ( p <0.05). The expression level of MNX1-AS1 in NSCLC cell line A549 was significantly higher than that in human normal lung epithelial cell line Beas-2B ( p <0.05). MNX1-AS1 expression was significantly associated with TNM stage and lymph node metastasis ( p <0.05). Kaplan-Meier survival curve analysis showed that high expression of MNX1-AS1 was associated with a poor prognosis in NSCLC. In addition, knockdown of MNX1-AS1 inhibited proliferation, migration and invasion of the NSCLC cell line A549 and promoted apoptosis. Conclusion: The up-regulation of LncRNA MNX1-AS1 is associated with the progression and prognosis of NSCLC. Knockdown of LncRNA MNX1-AS1 inhibits proliferation, migration and invasion of NSCLC cells and promotes apoptosis.