Open AccessModulation of Inflammation-Related Lipid Mediator Pathways by Celastrol During Human Macrophage Polarization
Author(s) -
Kehong Zhang,
Paul M. Jordan,
Simona Pace,
R. Hofstetter,
Markus Werner,
Xinchun Chen,
Oliver Werz
Publication year - 2022
Publication title -
journal of inflammation research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.656
H-Index - 33
ISSN - 1178-7031
DOI - 10.2147/jir.s356964
Subject(s) - chemistry , inflammation , macrophage polarization , tripterygium wilfordii , monocyte , macrophage , biochemistry , biology , immunology , in vitro , medicine , alternative medicine , pathology
Celastrol (CS) is a major active ingredient of the Chinese/Asian herb Tripterygium wilfordii that is frequently used as phytomedicine to treat inflammation and autoimmune diseases. We showed before that short-term exposure to CS (1 µM) favorably impacts the biosynthesis of inflammation-related lipid mediators (LM) in human polarized macrophages by modulating the activities of different lipoxygenases (LOXs). However, whether CS regulates the expression of LOXs and other related LM-biosynthetic enzymes during macrophage polarization is unknown. Here, we investigated how CS affects LM-biosynthetic enzyme expression on the protein level and studied concomitant LM signature profiles during polarization of human monocyte-derived macrophages (MDM) towards M1- and M2-like phenotypes.