Open AccessIn vitro Phenotype Induction of Circulating Monocytes: CD16 and CD163 Analysis
Author(s) -
Claudio Karsulovic,
Fabián Tempio,
Mercedes López,
Julia Guerrero,
Annelise Goecke
Publication year - 2021
Publication title -
journal of inflammation research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.656
H-Index - 33
ISSN - 1178-7031
DOI - 10.2147/jir.s292513
Subject(s) - cd14 , cd16 , cd163 , monocyte , peripheral blood mononuclear cell , flow cytometry , biology , immunology , intracellular , cd64 , interleukin 10 , microbiology and biotechnology , antigen , cytokine , macrophage , in vitro , biochemistry , cd3 , cd8
CD14 (monocyte differentiation antigen, LPS binding protein - endotoxin receptor) and CD16 (FcγRIII, Low-affinity receptor for IgG) define three subpopulations of circulating monocytes with different inflammatory and phagocytic capabilities. Contradictory reports exist regarding both in vivo monocyte phenotype-disease association and response of these circulating monocytes to in vitro stimulation. We analyzed phenotypic changes in circulating monocytes when stimulated with LPS (pro-inflammatory stimulus) and IL-4 (alternative inflammatory stimulus).