Open AccessThe Escalation of Osteosarcoma Stem Cells Apoptosis After the Co-Cultivation of Peripheral Blood Mononuclear Cells Sensitized with Mesenchymal Stem Cells Secretome and Colony Stimulating Factor-2 in vitro
Author(s) -
Ferdiansyah Mahyudin,
Fachrizal Arfani Prawiragara,
Mouli Edward,
Dwikora Novembri Utomo,
Muhammad Hardian Basuki,
Yunus Abdul Bari,
Alexander Patera Nugraha,
Fedik Abdul Rantam
Publication year - 2021
Publication title -
journal of blood medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.676
H-Index - 18
ISSN - 1179-2736
DOI - 10.2147/jbm.s305566
Subject(s) - mesenchymal stem cell , peripheral blood mononuclear cell , tumor necrosis factor alpha , annexin , medicine , apoptosis , osteosarcoma , cancer research , stem cell , immunology , fluorescein isothiocyanate , andrology , in vitro , pathology , biology , flow cytometry , microbiology and biotechnology , biochemistry , physics , quantum mechanics , fluorescence
Peripheral blood mononuclear cells (PBMCs) sensitized with mesenchymal stem cells (MSCs) secretome and/or colony stimulating factor-2 (CSF-2) as an immunotherapy candidate may escalate osteosarcoma stem cells (OS-SCs) apoptosis. This study aimed to investigate the escalation of osteosarcoma stem cells' apoptosis after the co-cultivation with PBMCs sensitized by MSCs secretome with/or CSF-2 and it was completed by analyzing the level of serum tumor necrosis factor-related apoptosis-inducing ligand (sTRAIL) and tumor necrosis factor-α (TNF-α) level, annexin V binding, caspase-3 and caspase-8 expression in vitro.