Open AccessOuter Membrane Vesicles Coating Nano-Glycyrrhizic Acid Confers Protection Against Borderella bronchiseptica Through Th1/Th2/Th17 Responses
Author(s) -
Yee Huang,
Nan Li,
Chi Xiao,
Jie Dong,
Ké Li,
Jvfen Cheng,
Quanan Ji,
Qiang Wang,
Guolian Bao,
Yan Liu
Publication year - 2022
Publication title -
international journal of nanomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.245
H-Index - 128
eISSN - 1178-2013
pISSN - 1176-9114
DOI - 10.2147/ijn.s350846
Subject(s) - immune system , bacterial outer membrane , in vivo , microbiology and biotechnology , materials science , biology , immunology , biochemistry , escherichia coli , gene
Outer membrane vesicles (OMVs) are spherical nano-sized proteolipids secreted by numerous pathogenic Gram-negative bacteria. Due to the immunostimulatory properties and protective efficacy, OMVs have received increasing attention as a candidate for the vaccine to prevent and treat bacterial infections. However, the immune response remains elusive due to the low structural stability and poor size homogeneity of the vesicles. In this study, OMVs were used to coat self-assembled glycyrrhizic acid nanoparticles (GANs) and obtain a stable OMV vaccine. The immunoprotective effects and anti-infection efficacy were evaluated in vivo and in vitro.