Open AccessAntisense vicR-Loaded Dendritic Mesoporous Silica Nanoparticles Regulate the Biofilm Organization and Cariogenicity of Streptococcus mutans
Author(s) -
Yuting Tian,
Yue Zhang,
Mengjiao Zhang,
Xianchun Chen,
Lei Lei,
Tao Hu
Publication year - 2022
Publication title -
international journal of nanomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.245
H-Index - 128
eISSN - 1178-2013
pISSN - 1176-9114
DOI - 10.2147/ijn.s334785
Subject(s) - streptococcus mutans , biofilm , plasmid , chemistry , recombinant dna , biology , microbiology and biotechnology , biochemistry , bacteria , gene , genetics
VicR is the essential response regulator related to the synthesis of exopolysaccharide (EPS) - one of the main cariogenic factors of S. mutans . An antisense vicR RNA (AS vicR ) could bind to vicR mRNA, hindering the transcription and translation of the vicR gene. We had constructed a recombinant plasmid containing the AS vicR sequence (plasmid-AS vicR ) and proved that it could reduce EPS synthesis, biofilm formation, and cariogenicity. However, the recombinant plasmids are supposed to be protected from enzymatic degradation and possess higher transformation efficiency. The principal objective of the present research was to construct an appropriate vector that can carry and protect the plasmid-AS vicR and investigate the effects of the carried plasmids on the cariogenicity of the S. mutans .