Open AccessPreservation of Small Extracellular Vesicle in Gelatin Methacryloyl Hydrogel Through Reduced Particles Aggregation for Therapeutic Applications
Author(s) -
Kelun Wu,
Chuan He,
Yue Wu,
Xiaojie Zhou,
Pan Liu,
Wei Tang,
Man Yu,
Weidong Tian
Publication year - 2021
Publication title -
international journal of nanomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.245
H-Index - 128
eISSN - 1178-2013
pISSN - 1176-9114
DOI - 10.2147/ijn.s334194
Subject(s) - self healing hydrogels , gelatin , angiogenesis , in vivo , human umbilical vein endothelial cell , biophysics , microbiology and biotechnology , umbilical vein , in vitro , chemistry , materials science , biology , biochemistry , cancer research , organic chemistry
Small extracellular vesicles (sEV) play an irreplaceable role in cell-cell communication. However, sEV in solution aggregate with each other during preservation, leading to impairment of the structures, contents, and functions of sEV. Therefore, there is a need to develop an optimal preservation method that combines high recovery rate, low cost, convenience, and easy-transportation in one. In this study, a new preservation strategy different from the cryopreservation or lyophilization was developed by reducing sEV particles aggregation.