Open AccessAn Isolation System to Collect High Quality and Purity Extracellular Vesicles from Serum
Author(s) -
Jian Yang,
Xin Gao,
Xiaotao Xing,
He Huang,
Qi Tang,
Shixing Ma,
Xun Xu,
Liang Cheng,
Maojiao Li,
Li Liao,
Weidong Tian
Publication year - 2021
Publication title -
international journal of nanomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.245
H-Index - 128
eISSN - 1178-2013
pISSN - 1176-9114
DOI - 10.2147/ijn.s328325
Subject(s) - extracellular vesicles , microvesicles , nanoparticle tracking analysis , chromatography , yield (engineering) , size exclusion chromatography , isolation (microbiology) , extracellular vesicle , materials science , chemistry , biology , bioinformatics , biochemistry , microbiology and biotechnology , enzyme , microrna , metallurgy , gene
Extracellular vesicles (EVs) are membrane-encapsulated nanoparticles that function as carriers and play a role in intercellular communication. There are a large number of EVs in the blood and serve as an indicator of pathophysiological conditions. Studies on the basics and application of EVs are hampered by the limitations of current protocols to isolate EVs from blood. However, current isolation methods are difficult to achieve a balance between yield and purity.