Open AccessIntracellular Delivery of Proteins into Living Cells by Low-Molecular-Weight Polyethyleneimine
Author(s) -
Yueheng Wu,
Lin Jiang,
Zixuan Dong,
Shaoxian Chen,
Xiyong Yu,
Shunqing Tang
Publication year - 2021
Publication title -
international journal of nanomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.245
H-Index - 128
eISSN - 1178-2013
pISSN - 1176-9114
DOI - 10.2147/ijn.s315444
Subject(s) - flow cytometry , intracellular , viability assay , cytotoxicity , chemistry , microbiology and biotechnology , cell , biochemistry , biology , in vitro
Intracellular protein delivery is emerging as a potential strategy to revolutionize therapeutics in the field of biomedicine, aiming at treating a wide range of diseases including cancer, inflammatory diseases and other oxidative stress-related disorders with high specificity. However, the current challenges and limitations are addressed to either synthetically or biologically through multipotency of engineering, such as protein modification, insufficient delivery of large-size proteins, deficiency or mutation of proteins, and high cytotoxicity.