Open AccessSerum Protects Cells and Increases Intracellular Delivery of Molecules by Nanoparticle-Mediated Photoporation
Author(s) -
Simple Kumar,
Eunice Lazau,
Carter Kim,
Naresh N. Thadhani,
Mark R. Prausnitz
Publication year - 2021
Publication title -
international journal of nanomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.245
H-Index - 128
eISSN - 1178-2013
pISSN - 1176-9114
DOI - 10.2147/ijn.s307027
Subject(s) - viability assay , intracellular , biophysics , fetal bovine serum , bovine serum albumin , nanoparticle , polyethylene glycol , chemistry , materials science , cell , nanotechnology , biochemistry , biology
Intracellular delivery of molecules is central to applications in biotechnology, medicine, and basic research. Nanoparticle-mediated photoporation using carbon black nanoparticles exposed to pulsed, near-infrared laser irradiation offers a physical route to create transient cell membrane pores, enabling intracellular delivery. However, nanoparticle-mediated photoporation, like other physical intracellular delivery technologies, necessitates a trade-off between achieving efficient uptake of exogenous molecules and maintaining high cell viability.