Open AccessEffects of Surface Charge, PEGylation and Functionalization with Dipalmitoylphosphatidyldiglycerol on Liposome–Cell Interactions and Local Drug Delivery to Solid Tumors via Thermosensitive Liposomes
Author(s) -
Matteo Petrini,
Wouter J.M. Lokerse,
Agnieszka Mach,
Martin Hossann,
Olivia M. Merkel,
Lars H. Lindner
Publication year - 2021
Publication title -
international journal of nanomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.245
H-Index - 128
eISSN - 1178-2013
pISSN - 1176-9114
DOI - 10.2147/ijn.s305106
Subject(s) - liposome , pegylation , nanocarriers , cationic liposome , peg ratio , biophysics , drug delivery , in vivo , doxorubicin , polyethylene glycol , chemistry , materials science , pharmacology , nanotechnology , biochemistry , transfection , medicine , biology , chemotherapy , microbiology and biotechnology , surgery , finance , economics , gene
Previous studies demonstrated the possibility of targeting tumor-angiogenic endothelial cells with positively charged nanocarriers, such as cationic liposomes. We investigated the active targeting potential of positively charged nanoparticles in combination with the heat-induced drug release function of thermosensitive liposomes (TSL). This novel dual-targeted approach via cationic TSL (CTSL) was thoroughly explored using either a novel synthetic phospholipid 1,2-dipalmitoyl- sn -glycero-3-phosphodiglycerol (DPPG 2 ) or a conventional polyethylene glycol (PEG) surface modification. Anionic particles containing either DPPG 2 or PEG were also included in the study to highlight difference in tumor enrichment driven by surface charge. With this study, we aim to provide a deep insight into the main differences between DPPG 2 - and PEG-functionalized liposomes, focusing on the delivery of a well-known cytotoxic drug (doxorubicin; DOX) in combination with local hyperthermia (HT, 41-43°C).