Open AccessLayer-Number-Dependent Effects of Graphene Oxide on the Pluripotency of Mouse Embryonic Stem Cells Through the Regulation of the Interaction Between the Extracellular Matrix and Integrins
Author(s) -
Guoxin Jing,
Kun Li,
Feiyue Sun,
Jintong Niu,
Rongrong Zhu,
Yechang Qian,
Shilong Wang
Publication year - 2021
Publication title -
international journal of nanomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.245
H-Index - 128
eISSN - 1178-2013
pISSN - 1176-9114
DOI - 10.2147/ijn.s301892
Subject(s) - embryonic stem cell , microbiology and biotechnology , embryoid body , leukemia inhibitory factor , extracellular matrix , stem cell , cellular differentiation , regenerative medicine , chemistry , integrin , in vitro , biophysics , biology , induced pluripotent stem cell , cell , biochemistry , gene
Embryonic stem cells (ESCs) possess great application prospects in biological research and regenerative medicine, so it is important to obtain ESCs with excellent and stable cellular states during in vitro expansion. The feeder layer culture system with the addition of leukemia inhibitory factor (LIF) is currently applied in ESC cultures, but it has a series of disadvantages that could influence the culture efficiency and quality of the ESCs. With the development of nanotechnology, many studies have applied nanomaterials to optimize the stem cell culture system and regulate the fate of stem cells. In this study, we investigated the layer-number-dependent biofunction of graphene oxide (GO) on the pluripotency of ESCs from mice (mESCs).