Open Access<p>Quality Assessment and Comparison of Plasma-Derived Extracellular Vesicles Separated by Three Commercial Kits for Prostate Cancer Diagnosis</p>
Author(s) -
Bairen Pang,
Ying Zhu,
Jie Ni,
Juanfang Ruan,
James Thompson,
David Malouf,
Joseph Bucci,
Peter Graham,
Yong Wang Li
Publication year - 2020
Publication title -
international journal of nanomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.245
H-Index - 128
eISSN - 1178-2013
pISSN - 1176-9114
DOI - 10.2147/ijn.s283106
Subject(s) - extracellular vesicles , prostate cancer , microvesicles , biomarker , medicine , cancer , prostate , exosome , extracellular vesicle , chromatography , chemistry , biology , microrna , biochemistry , gene , microbiology and biotechnology
Current standard biomarkers in clinic are not specific enough for prostate cancer (PCa) diagnosis. Extracellular vesicles (EVs) are nano-scale vesicles released by most mammalian cells. EVs are promising biomarker source for PCa liquid biopsy due to its minimal invasive approach, rich information and improved accuracy compared to the clinical standard prostate-specific antigen (PSA). However, current EV separation methods cannot separate pure EVs and the quality characteristics from these methods remain largely unknown. In this study, we evaluated the quality characteristics of human plasma-derived EVs by comparing three clinical suitable separation kits.