Open Access<p>Calcium-siRNA Nanocomplexes Optimized by Bovine Serum Albumin Coating Can Achieve Convenient and Efficient siRNA Delivery for Periodontitis Therapy</p>
Author(s) -
Xiawei Wei,
Wen Song,
Yi Cui,
Yang Zhang,
Shenglin Mei,
Qintao Wang
Publication year - 2020
Publication title -
international journal of nanomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.245
H-Index - 128
eISSN - 1178-2013
pISSN - 1176-9114
DOI - 10.2147/ijn.s278103
Subject(s) - biocompatibility , bovine serum albumin , confocal microscopy , biophysics , transfection , small interfering rna , materials science , chemistry , in vivo , biochemistry , microbiology and biotechnology , biology , gene , metallurgy
Reducing toxicity, immunogenicity, and costs of small interfering RNAs (siRNA) carrier materials are key goals for RNA interference (RNAi) technology transition from bench to bed. Recently, calcium ions (Ca 2+ ) have garnered attention as a novel, alternative material for delivering siRNA to cells. However, the tolerance for Ca 2+ concentration varies in different cell types, which has limited its applications in vivo. Bovine serum albumin (BSA) can bind to Ca 2+ through chelation. Moreover, BSA is a favorable coating material for nanoparticles owing to its excellent biocompatibility. Therefore, we hypothesized that coating Ca 2+ -siRNA with BSA helps buffer Ca 2+ toxicity in vivo.