Open Access<p>Development and screening of brain-targeted lipid-based nanoparticles with enhanced cell penetration and gene delivery properties</p>
Author(s) -
Bruna dos Santos Rodrigues,
Sushant Lakkadwala,
Takahisa Kanekiyo,
Jagdish Singh
Publication year - 2019
Publication title -
international journal of nanomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.245
H-Index - 128
eISSN - 1178-2013
pISSN - 1176-9114
DOI - 10.2147/ijn.s215941
Subject(s) - liposome , transfection , internalization , gene delivery , endocytosis , in vivo , blood–brain barrier , transferrin receptor , nanocarriers , biodistribution , biophysics , microbiology and biotechnology , cytotoxicity , drug delivery , chemistry , in vitro , transferrin , biochemistry , materials science , biology , cell , nanotechnology , neuroscience , gene , central nervous system
The potential of gene therapy for treatment of neurological disorders can be explored using designed lipid-based nanoparticles such as liposomes, which have demonstrated ability to deliver nucleic acid to brain cells. We synthesized liposomes conjugated to cell-penetrating peptides (CPPs) (vascular endothelial-cadherin-derived peptide [pVec], pentapeptide QLPVM and HIV-1 trans-activating protein [TAT]) and transferrin (Tf) ligand, and examined the influence of surface modifications on the liposome delivery capacity and transfection efficiency of encapsulated plasmid DNA. The design of liposomes was based on targeting molecular recognition of transferrin receptor overexpressed on the blood-brain barrier (BBB) with enhanced internalization ability of CPPs.