Open AccessSARS-CoV-2 Variants Detection Using TaqMan SARS-CoV-2 Mutation Panel Molecular Genotyping Assays
Author(s) -
Puja Neopane,
Jerome Nypaver,
Rojeet Shrestha,
Safedin Beqaj
Publication year - 2021
Publication title -
infection and drug resistance
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.033
H-Index - 39
ISSN - 1178-6973
DOI - 10.2147/idr.s335583
Subject(s) - taqman , genotyping , genotype , covid-19 , microbiology and biotechnology , virology , snp genotyping , single nucleotide polymorphism , biology , mutation , rna extraction , real time polymerase chain reaction , genetics , rna , medicine , gene , pathology , disease , infectious disease (medical specialty)
For rapid detection and tracking of SARS-CoV-2, a simple screening method alternative to laborious and expensive sequencing is highly desirable. Here, we evaluated performance characteristics of TaqMan SARS-CoV-2 mutation panel genotyping molecular assay for detection of most common reported SARS-CoV-2 variants using specific RT-PCR assays targeting single nucleotide polymorphisms (SNP).