Open AccessMultiplex Real-Time Polymerase Chain Reaction on Sputum for the Diagnosis of Pneumocystis jirovecii Pneumonia in Children: A Retrospective Study
Author(s) -
Juan Jiang,
Xia Wang,
Jian He,
Donglei Liao,
Xingming Deng
Publication year - 2021
Publication title -
infection and drug resistance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.033
H-Index - 39
ISSN - 1178-6973
DOI - 10.2147/idr.s326814
Subject(s) - pneumocystis jirovecii , pneumocystis pneumonia , polymerase chain reaction , medicine , sputum , multiplex polymerase chain reaction , pneumonia , multiplex , virology , microbiology and biotechnology , real time polymerase chain reaction , immunology , biology , pathology , bioinformatics , gene , tuberculosis , genetics
Pneumocystis jirovecii pneumonia (PCP) is a serious opportunistic infection in immunocompromised children. Real-time polymerase chain reaction (PCR) is widely used for the diagnosis of PCP due to its good accuracy. However, the diagnostic performance of multiplex real-time PCR on sputum in children with PCP has never been explored.