Open AccessDevelopment and Validation of Multiplex Quantitative PCR Assay for Detection of Helicobacter pylori and Mutations Conferring Resistance to Clarithromycin and Levofloxacin in Gastric Biopsy
Author(s) -
Hasyanee Binmaeil,
Alfizah Hanafiah,
Isa Mohamed Rose,
Raja Affendi Raja Ali
Publication year - 2021
Publication title -
infection and drug resistance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.033
H-Index - 39
ISSN - 1178-6973
DOI - 10.2147/idr.s325056
Subject(s) - clarithromycin , 23s ribosomal rna , helicobacter pylori , levofloxacin , multiplex polymerase chain reaction , real time polymerase chain reaction , multiplex , biology , microbiology and biotechnology , polymerase chain reaction , gene , antibiotics , genetics , ribosome , rna
More than half of the world's population is infected with Helicobacter pylori , which can cause chronic gastritis. WHO has regarded clarithromycin-resistant H. pylori as a high priority pathogen. Hence, accurate diagnosis and detection of clarithromycin- and levofloxacin-resistant H. pylori strains is essential for proper management of infection. The objective of this study was to develop and optimize multiplex quantitative PCR assay for detection of mutations associated with clarithromycin and levofloxacin resistance in H. pylori directly from the gastric biopsies.