Open AccessFluorescence in situ Hybridization (FISH) in the Microbiological Diagnostic of Deep Sternal Wound Infection (DSWI)
Author(s) -
Nick Spindler,
Annette Moter,
Alexandra Wießner,
Tanja Gradistanac,
Michael A. Borger,
Arne C. Rodloff,
Stefan Langer,
Judith Kikhney
Publication year - 2021
Publication title -
infection and drug resistance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.033
H-Index - 39
ISSN - 1178-6973
DOI - 10.2147/idr.s310139
Subject(s) - mediastinitis , medicine , fish <actinopterygii> , surgery , complication , in situ hybridization , microbiology and biotechnology , biology , fishery , biochemistry , gene expression , gene
Postoperative mediastinitis after cardiac surgery is still a devastating complication. Insufficient microbiological specimens obtained by superficial swabbing may only detect bacteria on the surface, but pathogens that are localized in the deep tissue may be missed. The aim of this study was to analyze deep sternal wound infection (DSWI) samples by conventional microbiological procedures and fluorescence in situ hybridization (FISH) in order to discuss a diagnostic benefit of the culture-independent methods and to map spatial organization of pathogens and microbial biofilms in the wounds.