Open Access<p>Guidelines and Considerations for Metabolic Tolerance Tests in Mice</p>
Author(s) -
Raquel Benedé-Ubieto,
Olga Estévez-Vázquez,
Pierluigi Ramadori,
Francisco Javier Cubero,
Yulia A. Nevzorova
Publication year - 2020
Publication title -
diabetes, metabolic syndrome and obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.853
H-Index - 43
ISSN - 1178-7007
DOI - 10.2147/dmso.s234665
Subject(s) - glucose homeostasis , diabetes mellitus , medicine , insulin , intensive care medicine , computer science , insulin resistance , endocrinology
The epidemic of the century, Diabetes Mellitus (DM) is continuously rising. Intensive research is urgently needed whereby experimental models represent an essential tool to optimise the diagnostic strategy and to improve therapy. In this review, we describe the central principles of the metabolic tests available in order to study glucose and insulin homeostasis in mice, focusing on the most widely used - the glucose and insulin tolerance tests. We provide detailed experimental procedures as well as the practical implementation of these methods and discuss the main factors that should be taken into account when using this methodology.