Open Access<p>Development and Validation of UHPLC-MS/MS Assay for Therapeutic Drug Monitoring of High-dose Methotrexate in Children with Acute Lymphoblastic Leukemia</p>
Author(s) -
Lejing Lian,
Bingcheng Lin,
Xiao Cui,
He Jie,
Zhe Wang,
Xiaodong Lin,
Wei-jian Ye,
Ruijie Chen,
Sun Wei
Publication year - 2020
Publication title -
drug design, development and therapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.964
H-Index - 64
ISSN - 1177-8881
DOI - 10.2147/dddt.s271568
Subject(s) - methotrexate , therapeutic drug monitoring , medicine , lymphoblastic leukemia , drug , pharmacology , leukemia
Precise and timely detection of methotrexate (MTX) concentration played a key role in high-dose MTX individualization therapy in acute lymphoblastic leukemia (ALL) children to avoid serious adverse effects or nonresponse. This report described a sensibility and validation of ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for therapeutic drug monitoring (TDM) of methotrexate concentration in children's plasma.