Open Access<p>Enhancing the Butyrylcholinesterase Activity in HEK-293 Cell Line by Dual-Promoter Vector Decorated on Lipofectamine</p>
Author(s) -
Vida Mirzaie,
Touba Eslaminejad,
Homayoon Babaei,
Seyed Noureddin NematollahiMahani
Publication year - 2020
Publication title -
drug design, development and therapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.964
H-Index - 64
ISSN - 1177-8881
DOI - 10.2147/dddt.s260419
Subject(s) - lipofectamine , hek 293 cells , transfection , microbiology and biotechnology , plasmid , butyrylcholinesterase , chemistry , cell culture , recombinant dna , gene , biology , enzyme , biochemistry , vector (molecular biology) , genetics , aché , acetylcholinesterase
Human butyrylcholinesterase (BChE) serves as a bio scavenger to counteract organophosphate poisoning. It is also a potential drug candidate in several therapeutic fields. Therefore, in the present study, we constructed a new dual-promoter plasmid consisting of Cytomegalovirus (CMV) and human elongation factor 1α (EF-1α) promoters and transfected that into HEK-293 cells using Lipofectamine to enhance the BChE secretion.