Open Access<p><em>N</em>-Acetyl-l-Leucine-Polyethyleneimine-Mediated Delivery of CpG Oligodeoxynucleotides 2006 Inhibits RAW264.7 Cell Osteoclastogenesis</p>
Author(s) -
Huining Wang,
Wenwen Yu,
Hongyan Li,
Yuanyuan Zheng,
Zhe Chen,
Hongbing Lin,
Yunzhong Shen
Publication year - 2020
Publication title -
drug design, development and therapy
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.964
H-Index - 64
ISSN - 1177-8881
DOI - 10.2147/dddt.s241826
Subject(s) - viability assay , microbiology and biotechnology , mtt assay , cpg oligodeoxynucleotide , apoptosis , transfection , chemistry , flow cytometry , biology , biochemistry , dna methylation , gene expression , gene
CpG oligodeoxynucleotides (CpG ODN) play important roles in resisting inflammation and bone resorption. However, the inherent instability and rapid degradation hinder their wider application. This study aimed to evaluate whether N -acetyl-L-leucine-modified polyethyleneimine ( N -Ac-L-Leu-PEI) could effectively deliver CpG ODN 2006 to RAW264.7 cells and and if it can regulate osteoclastogenesis in vitro.