Open AccessA Sensitive PCR-Based Method for Somatic Mutations Enrichment and Screening
Author(s) -
Yaming Xiong,
Hailing Tang
Publication year - 2021
Publication title -
cancer management and research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.024
H-Index - 40
ISSN - 1179-1322
DOI - 10.2147/cmar.s335679
Subject(s) - cold pcr , kras , exon , biology , mutation , microbiology and biotechnology , mutant , gene , dna , germline mutation , genetics , point mutation
EGFR and KRAS are the most frequently mutated genes in lung cancers, occurring in about 60% of all cases. Mutation genes assay has emerged as a promising blood-based biomarker for monitoring cancer dynamics noninvasively. However, detection can be challenging in patients where plasma often contains low levels of tumor-derived DNA fragments.