Open AccessThe Predominant Prognostic Significance of NOTCH1 Mutation Defined by Emulsion PCR in Chronic Lymphocytic Leukemia
Author(s) -
Katarzyna Skórka,
Michał Chojnacki,
Marta Masternak,
Agnieszka Karczmarczyk,
Edyta Subocz,
Ewa Wawrzyniak,
Krzysztof Giannopoulos
Publication year - 2021
Publication title -
cancer management and research
Language(s) - English
Resource type - Journals
ISSN - 1179-1322
DOI - 10.2147/cmar.s302245
Subject(s) - ighv@ , oncology , medicine , proportional hazards model , chronic lymphocytic leukemia , multivariate analysis , survival analysis , hazard ratio , digital polymerase chain reaction , biology , leukemia , polymerase chain reaction , genetics , confidence interval , gene
NOTCH1 mut represents a new prognostic marker in chronic lymphocytic leukaemia (CLL). The low sensitivity of the current methods may increase the risk of false-negative results, particularly in patients with low NOTCH1 mut allelic burden. This study compared two methods of the NOTCH1 mut assessment including droplet digital PCR (ddPCR) and amplification-refractory mutation system PCR (ARMS-PCR) untreated CLL patients.