Open Access<p>A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles from Culture Supernatant of Human Ovarian Cancer Cell Line A2780 and Body Fluids of High-Grade Serous Carcinoma Patients</p>
Author(s) -
Ruili Jiao,
Shipeng Sun,
Xiaoqiong Gao,
Ran Cui,
Guangming Cao,
Huali Wei,
Shuzhen Wang,
Zhenyu Zhang,
Huimin Bai
Publication year - 2020
Publication title -
cancer management and research
Language(s) - English
Resource type - Journals
ISSN - 1179-1322
DOI - 10.2147/cmar.s228288
Subject(s) - cd63 , cell culture , nanoparticle tracking analysis , chemistry , polyethylene glycol , serous fluid , microbiology and biotechnology , differential centrifugation , cell , ovarian cancer , biology , cancer , biochemistry , microvesicles , microrna , genetics , gene
This study tried to evaluate whether 8% polyethylene glycol (PEG) 6000 precipitation combined with differential ultracentrifugation (PPDU) was an efficient and practical method for the enrichment and purification of extracellular vesicles (EVs) derived from the culture supernatant of human ovarian cancer cell line A2780 and from body fluids of patients with high-grade serous carcinoma (HGSC).